Simultaneous Quantitative Determination of Ustiloxin A and Ustiloxin D in Rice  Grains by High Performance Liquid Chromatographytandem Mass Spectrometry

doi:10.3969/j.issn.10017216.2012.02.015

Chinese Journal of Rice Science ›› 2012, Vol. 26 ›› Issue (2): 246-250.DOI: 10.3969/j.issn.10017216.2012.02.015

• Experimental Techniques • Previous Articles Next Articles

Simultaneous Quantitative Determination of Ustiloxin A and Ustiloxin D in Rice Grains by High Performance Liquid Chromatographytandem Mass Spectrometry

JI Fang, CAO Huan, XU Jianhong, YIN Xianchao, SHI Jianrong*

Key Laboratory of Food Quality and Safety of Jiangsu Province/State Key Laboratory Breeding Base for Jiangsu Province/Key Laboratory of AgroFood Safety and Quality, Ministry of Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing 210014， China；

Received:2011-06-27 Revised:2011-10-14 Online:2012-03-10 Published:2012-03-10
Contact: SHI Jianrong*

高效液相色谱串联质谱法定量检测稻谷中的稻曲病菌毒素A和D

祭芳,曹欢,徐剑宏,殷宪超,史建荣*

江苏省农业科学院江苏省食品质量安全重点实验室/省部共建国家重点实验室培育基地/农业部食品安全监控重点开放实验室，江苏南京 210014;

通讯作者: 史建荣*
基金资助:
国家自然科学基金资助项目（30901006）；国家重大科技专项（2009ZX08011015B, 2008ZX08011003）; 江苏省科技支撑计划资助项目（BE2010757）

Abstract

Abstract: A method based on high performance liquid chromatography coupled with tandem mass spectrometry(HPLCMS/MS) was developed for simultaneous determination of ustiloxin A and ustiloxin D. This method is simple，sensitive and accurate, and can be used for simultaneous quantitative determination of ustiloxin A and ustiloxin D in rice grains.

Key words: high performance liquid chromatographytandem mass spectrometry(HPLCMS), ustiloxin A, ustiloxin D, rice grain

摘要： 建立了稻谷中稻曲病菌毒素A和D污染物的高效液相色谱串联质谱的分析方法。该方法简单、灵敏、准确，可同时定量检测稻谷中稻曲病菌毒素A和稻曲病菌毒素D。

关键词: 高效液相色谱串联质谱法, 稻曲病菌毒素A, 稻曲病菌毒素D, 稻谷

CLC Number:

S4351114+9

JI Fang, CAO Huan, XU Jianhong, YIN Xianchao, SHI Jianrong*. Simultaneous Quantitative Determination of Ustiloxin A and Ustiloxin D in Rice Grains by High Performance Liquid Chromatographytandem Mass Spectrometry[J]. Chinese Journal of Rice Science, 2012, 26(2): 246-250.

祭芳,曹欢,徐剑宏,殷宪超,史建荣*. 高效液相色谱串联质谱法定量检测稻谷中的稻曲病菌毒素A和D[J]. 中国水稻科学, 2012, 26(2): 246-250.

References

\[1\]陈永永. 我国稻曲病研究概况. 中国农学通报, 1992, 8(4): 1519.

\[2\]王伟刚, 郑明, 谢春甫, 等. 水稻稻曲病流行危害分析及防治对策. 现代农业科学, 2009,16(3):63165.

\[3\] 黄世文, 余柳青. 国内稻曲病的研究现状. 江西农业学报, 2002,14 (2): 4551.

\[4\]Koiso Y, Natori M, Iwasaki S, et al. Ustiloxin: A phytotoxin and a mycotoxin from false smult halls on rice panicles. Tetr Lett, 1992, 33(29): 41574160.

\[5\]Koiso Y, Iwasaki Y, Hanaoka S, et al. Ustiloxins, antimitotic cyclic peptides from false smut balls on rice panicles caused by Ustilaginoidea virens. J Antib, 1994, 47(7): 765773.

\[6\]Suwa M. Study on Indakouji. Part Ⅰ. JAMAS, 1915,13: 661686.

\[7\] Koiso Y, Morisaki N, Yamashita Y, et al. Isolation and structure of an antimitotic cyclic peptide, ustiloxin F: Chemical interrelation with a homologous peptide, ustiloxin B. J Antibi, 1998, 51(4):41822.

\[8\] Evans L P, Wu C D, Cao Y, et al. Evolution of the total syntheses of ustiloxin natural products and their analogues. J Amer Chem Soc, 2008, 130(7): 23512364.

\[9\]Jordan M A. Mechanism of action of antitumor drugs that interact with microtubules and tubulin. Curr MediC Chem  AntiCan Agen, 2002, 2(17): 117.

\[10\]Nakamura K, Izumiyama N, Ohtsubo K, et al. “Lupinosis”like lesions in mice caused by ustiloxin, produced by Ustilaginoieda virens: A morphological study. Natural Toxins, 1994, 2(1): 2228.

\[11\]Li Y, Koiso Y, Kobayashi H, et al. Ustiloxins, new antimitotic cyclic peptides: Interaction with porcine brain tubulin. Biocheml Pharmacol, 1995, 49(10): 13671372.

\[12\]Iwasaki S. Chemistry and biological activity of the mycotoxins interfering with tubulin function. Proc Jpn Assoc Mycot, 1992, 35: 4143.

\[13\]Hamel E. Interactions of antimitotic peptides and depsipeptides with tubulin. Biopolymers, 2002, 66(3): 14260.

\[14\]Nakamura K, Izumiyama N, Ohtsubo K. Apoptosis induced in the liver, kidney and urinary bladder of mice by the fungal toxin produced by Ustilaginoidea virens. Mycotoxins, 1993, 38: 2530.

\[15\]Miyazaki S, Matsumoto Y, Uchihara T, et al. Highperformance liquid chromatographic determination of ustiloxin A in forage rice silage. J Vet Med Sci, 2009, 71(2): 239241.

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Simultaneous Quantitative Determination of Ustiloxin A and Ustiloxin D in Rice Grains by High Performance Liquid Chromatographytandem Mass Spectrometry

高效液相色谱串联质谱法定量检测稻谷中的稻曲病菌毒素A和D

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